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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
13/01/2023 |
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Data da última atualização: |
13/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, E. R.; FREIRE, R. P.; SUZUKI, M. F.; OLIVEIRA, J. E.; YOSIDAKI, V. L.; PERONI, C. N.; SEVILHANO, T.; ZORZETO, M.; TORATI, L. S.; SOARES, C. R. J.; LIMA, I. D. de M.; KRONENBERGER, T.; MALTAROLLO, V. G.; BARTOLINI, P. |
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Afiliação: |
ELIANA ROSA LIMA, IPEN-CNEN; RENAN PASSOS FREIRE, IPEN-CNEN; MIRIAM FUSSAE SUZUKI, IPEN-CNEN; JOÃO EZEQUIEL OLIVEIRA, IPEN-CNEN; VANESSA LUNA YOSIDAKI, IPEN-CNEN; CIBELE NUNES PERONI, IPEN-CNEN; THAÍS SEVILHANO, IPEN-CNEN; MOISÉS ZORZETO, PISCICULTURA RAÇA, MT; LUCAS SIMON TORATI, CNPASA; CARLOS ROBERTO JORGE SOARES, IPEN-CNEN; IGOR DANIEL DE MIRANDA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; THALES KRONENBERGER, Eberhard Karls University Tübingen, Germany; VINICIUS GONÇALVES MALTAROLLO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PAOLO BARTOLINI, IPEN-CNEN. |
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Título: |
Isolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomolecules, v. 13, n. 1, art. 158, 2023. |
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ISSN: |
2218-273X |
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DOI: |
https://doi.org/10.3390/biom13010158 |
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Idioma: |
Inglês |
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Conteúdo: |
In a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies. |
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Palavras-Chave: |
Growth hormone; In silico sequencing. |
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Thesagro: |
Hormônio Animal; Peixe; Pirarucu. |
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Thesaurus Nal: |
Arapaima gigas; Hormones; Molecular models. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150987/1/biomolecules-2023.pdf
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Marc: |
LEADER 02661naa a2200397 a 4500
001 2150987
005 2023-02-13
008 2023 bl uuuu u00u1 u #d
022 $a2218-273X
024 7 $ahttps://doi.org/10.3390/biom13010158$2DOI
100 1 $aLIMA, E. R.
245 $aIsolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH).$h[electronic resource]
260 $c2023
520 $aIn a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies.
650 $aArapaima gigas
650 $aHormones
650 $aMolecular models
650 $aHormônio Animal
650 $aPeixe
650 $aPirarucu
653 $aGrowth hormone
653 $aIn silico sequencing
700 1 $aFREIRE, R. P.
700 1 $aSUZUKI, M. F.
700 1 $aOLIVEIRA, J. E.
700 1 $aYOSIDAKI, V. L.
700 1 $aPERONI, C. N.
700 1 $aSEVILHANO, T.
700 1 $aZORZETO, M.
700 1 $aTORATI, L. S.
700 1 $aSOARES, C. R. J.
700 1 $aLIMA, I. D. de M.
700 1 $aKRONENBERGER, T.
700 1 $aMALTAROLLO, V. G.
700 1 $aBARTOLINI, P.
773 $tBiomolecules$gv. 13, n. 1, art. 158, 2023.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
21/05/2009 |
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Data da última atualização: |
02/08/2019 |
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Autoria: |
BEUTLER, A. N.; CENTURION, J. F.; SILVA, A. P. da; CENTURION, M. A. P. da C.; LEONEL, C. L.; FREDDI, O. da S. |
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Afiliação: |
Amauri Nelson Beutler, Universidade Federal do Pampa/Centro de Ciências Agrárias; José Frederico Centurion, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias - FCAV/Departamento de Solos e Adubos; Alvaro Pires da Silva, Universidade de São Paulo/Escola Superior de Agricultura Luiz de Queiroz/Departamento de Ciência do Solo; Maria Aparecida Pessôa da Cruz Centurion, Unesp/FCAV/Departamento de Produção Vegetal; Cristian Luarte Leonel, Unesp/FCAV/Departamento de Produção Vegetal; Onã da Silva Freddi, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias - FCAV/Departamento de Solos e Adubos. |
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Título: |
Soil compaction by machine traffic and least limiting water range related to soybean yield. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 11, p. 1591-1600, nov. 2008. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Compactação do solo por tráfego de máquinas e intervalo hídrico ótimo na produtividade de soja. |
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Conteúdo: |
The research aimed to evaluate machine traffic effect on soil compaction and the least limiting water range related to soybean cultivar yields, during two years, in a Haplustox soil. The six treatments were related to tractor (11 Mg weight) passes by the same place: T0, no compaction; and T1*, 1; T1, 1; T2, 2; T4, 4 and T6, 6. In the treatment T1*, the compaction occurred when soil was dried, in 2003/2004, and with a 4 Mg tractor in 2004/2005. Soybean yield was evaluated in relation to soil compaction during two agricultural years in completely randomized design (compaction levels); however, in the second year, there was a factorial scheme (compaction levels, with and without irrigation), with four replicates represented by 9 m2 plots. In the first year, soybean [Glycine max (L.) Merr.] cultivar IAC Foscarim 31 was cultivated without irrigation; and in the second year, IAC Foscarim 31 and MG/BR 46 (Conquista) cultivars were cultivated with and without irrigation. Machine traffic causes compaction and reduces soybean yield for soil penetration resistance between 1.64 to 2.35 MPa, and bulk density between 1.50 to 1.53 Mg m-3. Soil bulk density from which soybean cultivar yields decrease is lower than the critical one reached at least limiting water range (LLWR =0). |
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Palavras-Chave: |
Glicine max; qualidade física do solo; soil physical quality. |
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Thesagro: |
Manejo do Solo. |
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Thesaurus NAL: |
soil management. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/106160/1/Soil-compaction.pdf
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Marc: |
LEADER 02169naa a2200253 a 4500
001 1125679
005 2019-08-02
008 2008 bl uuuu u00u1 u #d
100 1 $aBEUTLER, A. N.
245 $aSoil compaction by machine traffic and least limiting water range related to soybean yield.
260 $c2008
500 $aTítulo em português: Compactação do solo por tráfego de máquinas e intervalo hídrico ótimo na produtividade de soja.
520 $aThe research aimed to evaluate machine traffic effect on soil compaction and the least limiting water range related to soybean cultivar yields, during two years, in a Haplustox soil. The six treatments were related to tractor (11 Mg weight) passes by the same place: T0, no compaction; and T1*, 1; T1, 1; T2, 2; T4, 4 and T6, 6. In the treatment T1*, the compaction occurred when soil was dried, in 2003/2004, and with a 4 Mg tractor in 2004/2005. Soybean yield was evaluated in relation to soil compaction during two agricultural years in completely randomized design (compaction levels); however, in the second year, there was a factorial scheme (compaction levels, with and without irrigation), with four replicates represented by 9 m2 plots. In the first year, soybean [Glycine max (L.) Merr.] cultivar IAC Foscarim 31 was cultivated without irrigation; and in the second year, IAC Foscarim 31 and MG/BR 46 (Conquista) cultivars were cultivated with and without irrigation. Machine traffic causes compaction and reduces soybean yield for soil penetration resistance between 1.64 to 2.35 MPa, and bulk density between 1.50 to 1.53 Mg m-3. Soil bulk density from which soybean cultivar yields decrease is lower than the critical one reached at least limiting water range (LLWR =0).
650 $asoil management
650 $aManejo do Solo
653 $aGlicine max
653 $aqualidade física do solo
653 $asoil physical quality
700 1 $aCENTURION, J. F.
700 1 $aSILVA, A. P. da
700 1 $aCENTURION, M. A. P. da C.
700 1 $aLEONEL, C. L.
700 1 $aFREDDI, O. da S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 11, p. 1591-1600, nov. 2008.
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Embrapa Unidades Centrais (AI-SEDE) |
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